development and characterization of a bioactive acellular matrix for bladder augmentation. by Allison Louise Brown

Cover of: development and characterization of a bioactive acellular matrix for bladder augmentation. | Allison Louise Brown

Published .

Written in English

Read online

About the Edition

In this work, a porcine derived bladder acellular matrix (BAM) was investigated as a potential material for bladder augmentation. The in vivo response of BAM was evaluated in a porcine bladder augmentation model. Achievement of bladder compliance was assessed through the use of mechanical tensile testing of native and BAM tissue explants out to 22 weeks post-operative. A significant decrease in rupture strains, combined with a significant increase in tissue moduli for the explanted grafts provided quantitative evidence for a fibroproliferative tissue response. Histological observations supported mechanical property data. Central regions of the grafts displayed limited urothelial coverage and newly developed smooth muscle was limited within a dense fibrotic collagen matrix.Following in vitro characterization, BAM was modified to include the glycosaminoglycan hyaluronan (HA) and a biologically active peptide derived from the matricellular protein Secreted Protein, Acidic, Rich in Cysteine (SPARC), termed SP4.2. A thiol-modified form of HA was incorporated and cross-linked into BAM to produce a disulfide cross-linked HA/BAM construct. When utilized in the co-culture model, the HA/BAM scaffold exhibited a significantly higher degree of matrix contraction and gelatinase activity compared to unmodified BAM. In contrast, addition of SP4.2 to the BAM-only scaffold produced a negligible effect on contraction. Gelatinase activity was however significantly reduced. The trend of reduced gelatinase activity was consistent for HA+SP4.2 modified BAM. Unlike the BAM+SP4.2 scaffold, matrices containing both molecules displayed significant increases in matrix contraction compared to BAM alone. These in vitro results suggest that both HA and SP4.2 have potential to modify the processes associated with bladder repair following bladder augmentation with BAM.In order to address these unfavorable outcomes, an in vitro model for studying BAM-mediated fibroproliferative events was developed. Methods for culturing primary porcine bladder smooth muscle (SMC) and urothelial cells (UEC) were established and cell seeding experiments demonstrated the ability of BAM to support the growth of these cell types. SMC-mediated matrix contraction, as well as SMC invasiveness into BAM, was enhanced in co-culture with UECs. The contractile and invasive capacity of co-cultured SMCs appeared to be, at least partially, attributable to elevated levels of cell-secreted gelatinase activity.

Book details

The Physical Object
Pagination222 leaves.
Number of Pages222
ID Numbers
Open LibraryOL19475404M
ISBN 100494027002

Download development and characterization of a bioactive acellular matrix for bladder augmentation.

Development of a Porcine Bladder Acellular Matrix with Well-Preserved Extracellular Bioactive Factors for Tissue Engineering February Tissue Engineering Part C Methods 16(5)   An ECM scaffold derived from the porcine urinary bladder refers to bladder acellular matrix (BAM) or bladder acellular matrix graft (BAMG), one of the most representative decellularized tissues.

BAM has been demonstrated to preserve bioactive factors well to support tissue regeneration and exhibits good biocompatibility.Cited by: 7. Bladder acellular matrices (ACMs) derived from natural tissue are gaining increasing attention for their role in tissue engineering and regeneration.

Unlike conventional scaffolds based on biodegradable polymers or gels, ACMs possess native biomechanical and many acquired biologic by: For an appropriate preservation of the endogenous bioactive properties of the bladder acellular matrix (BAM), critical steps have to be included in the decellularization process, like, for.

Development and Characterization of Acellular Extracellular Matrix Scaffolds from Porcine Menisci for Use in Cartilage Tissue Engineering while retaining the maximum amount of bioactive. Biomaterials including acellular bladder matrix, small intestinal submucosa (SIS), and poly-glycolic acid (PGA), either alone or seeded with primary or progenitor bladder cell sources, have been.

Development and Characterization of Acellular Extracellular Matrix Scaffolds from Porcine Menisci for Use in Cartilage Tissue Engineering Ying-Chen Chen, PhD, 1 Ray-Neng Chen, PhD, 2 Hua-Jing Jhan, PhD, 1 Der-Zen Liu, PhD, 3, 4 Hsiu-O Ho, PhD, 1 Yong Mao, PhD, 5 Joachim Kohn, PhD, 5, 6 and Ming-Thau Sheu, PhD 1, 7.

Work in our laboratory has focused on bladder acellular matrix (BAM) as a scaffold for in vivo bladder tissue engineering. BAM is derived from full-thickness porcine bladder; the scaffold is produced by a detergent and enzymatic extraction process which removes all cellular constituents from the bladder extracellular matrix.

Development and characterization of an acellular porcine cartilage bone matrix for use in this study has generated data on the production of an acellular cartilage bone matrix scaffold for use in osteochondral defect repair.

jejunum, and urinary bladder in rat subcutaneous model, Journal of Biomedical Materials Research Part B: Applied. Nevertheless, the new commercial bladder acellular matrix has been found to have better immune response. Addition of stem cells can help to modulate the immune response to acellular scaffolds.

Although rare, there is a risk of infection with prion viruses especially with SIS. Bladder Acellular Matrix Preparation Considered pioneers of acellular matrices, Meezan et al.

are valuable scienti c gures who proposed simple and versatile techniques for biological matrices decellularization and suggested their usage in regenerative medicine [ ]. Since that time, there have been several di erent types of acellular matrices.

In this study, we compared four decellularization protocols and finally developed an optimized one through which a porcine bladder acellular matrix (BAM) with well-preserved extracellular bioactive factors had been prepared. The aim of this study was to evaluate a novel asymmetric bilayer chitosan scaffold compared with conventional bladder acellular matrix graft (BAMG) in a rat model of bladder augmentation.

Identification and characterization of bioactive factors in bladder submucosa matrix A.L. Brown et al. 22 week assessment of bladder acellular matrix as a bladder augmentation material in a porcine model Horst et al. fabricated hybrid scaffolds via electrospinning of PLGA microfibers directly onto surface of a bladder acellular matrix.

This is why bladder acellular matrix graft (BAMG) has been also tested. However, porcine bladder augmentation with a seeded BAMG demonstrated a local cells infiltration which remained limited in the periphery. The insufficient cellular organization resulted in.

Preparation and characterization of acellular adipose tissue matrix using a combination of physical and chemical treatments their presence within the matrix provides a possible avenue for bioactive molecule delivery Kearney JN, Watterson KG, Fisher J, Ingham E.

Development and characterization of an acellular human pericardial matrix. Most attempts have not been successful because of mechanical, structural, functional, or biocompatibility problems. Bladder acellular matrix (BAM) is obtained by removing cellular components from donor bladders, leaving a tissue matrix consisting of collagen, elastin, fibronectin, glycosaminoglycans, proteoglycans, and growth factors.

Bladder acellular matrix (BAM) is an extensively studied scaffold that has been utilized to support bladder augmentation in various animal models and several clinical trials 4,5. The objective of this study was to investigate the feasibility of bladder acellular matrix grafts (BAMGs) seeded with adipose-derived stem cells (ASCs) followed by intraperitoneal incubation for bladder reconstruction in a rat model of bladder augmentation, and to explore the underlying mechanism.

Due to these requirements, the ideal proposition seems to be extracellular matrix-derived grafts, like bladder acellular matrix (BAM). BAM is the three-dimensional scaffold of extracellular matrix (ECM) origin. It is composed of typical ECM constituents, which impart to this biomaterial the required biocompatibility and mechanical properties.

Development and characterization of an acellular porcine cartilage bone matrix for use in tissue engineering 19 August | Journal of Biomedical Materials Research Part A, Vol. 99A, No. 2 The knee meniscus: Structure–function, pathophysiology, current repair techniques, and prospects for regeneration.

Bladder acellular matrix (BAM) as an extracellular matrix (ECM) scaffold has already been successfully used both experimentally and clinically for urethral reconstruction [4–6]. However, BAM generally exhibits weak adjustability of property in the preparation process and high density of collagen hindering cells penetration.

Objective To determine the decreased antigenicity of the bladder acellular matrix graft (BAMG) through xenotransplantation and to assess the in vivo and in vitro functional properties of the rat u. Currently the standard surgical treatment for bladder defects is augmentation cystoplasty with autologous tissues, which has many side effects.

Biomat. The complication rates of proximal hypospadias, especially fistula, are much higher than those of distal hypospadias. Urethral coverage is an effective method for reducing fistulas.

Acellular dermal matrix (ADM) has been shown to exhibit structural compatibility and biocompatibility, both of which promote tissue healing. The present non-randomized study evaluated the efficiency, feasibility. Brown AL, Farhat W, Merguerian PA, Wilson GJ, Khoury AE, Woodhouse KA: 22 week assessment of bladder acellular matrix as a bladder augmentation material in a porcine model.

Biomaterials– /S(01) Article Google Scholar. In this group of biomaterials, the bladder acellular matrix graft (BAMG) has recently been reported to be successful in rats for partial bladder replacement [ 7 8 9].

The BAMG provides complete regeneration of all wall components, and in vitro studies of matrix‐regenerated muscle strips show ≈50% contractility when compared with host strips. CONCLUSIONS: Bladder regeneration was feasible in these patients, but bladder capacity and compliance was poorly increased to obtain significant clinical benefit.

Histology showed poor muscle components. The acellular matrix grafting failed to provide long-term effective results in terms of continence achievement. To evaluate if the implantation of bilateral ureters into a bladder acellular matrix graft (BAMG) at the time of its implantation would enhance bladder regeneration in a partial substitution BAMG.

MATERIALS AND METHODS • Partial cystectomies were performed under general anaesthesia in 12 pigs, followed by augmentation with a BAMG. Bladder acellular matrix and acellular blood vessels have recently been applied with the promising outcomes, but these outcomes may also be obtained by the use of stem cells and pre‐implantation methods, something that was not performed in combination with SIS to obtain the ureter (Jack et al., ).

Amniotic membrane. The bladder acellular matrix (BAM) derived from porcine is a frequently used biomaterial with the compositional and structural characteristics similar to native urinary extracellular matrix.

Zhe Z, Jun D, Yang Z, Mingxi X, Ke Z, Ming Z, et al. Bladder acellular matrix grafts seeded with adipose-derived stem cells and incubated intraperitoneally promote the regeneration of bladder smooth muscle and nerve in a rat model of bladder augmentation.

Stem Cells Dev. () – doi: /scd Yang B, Zhang Y, Zhou L, et al. Development of a porcine bladder acellular matrix with well-preserved extracellular bioactive factors for tissue engineering. Tissue Eng C Meth ; – [ PubMed ]. bladder augmentation. At last, the induced bladder tissue contained all 3 layers of the normal bladder, and was functionally contractile.

Both studies, in which the acellular matrix was used to repair tissue defect directly, have shown that the acellular matrix could induce specific tissue regeneration in.

Generally, three classes of biomaterials are used for tissue engineering purposes: acellular tissue matrices, natural polymers, and synthetic polymers [3]. Body organs are complex structures composed of extracellular matrix (ECM) and cell ingredients.

Song L. et al. Bladder acellular matrix and its application in bladder augmentation. Tissue Eng Part B – (). Bolland F. et al. Development and characterisation of a full-thickness acellular porcine bladder matrix for tissue engineering. Biomateri – (). A preliminary experience on in vivo bladder wall regeneration in a subset of patients born with exstrophy–epispadias complex is reported.

The objective was to improve bladder capacity and compliance without bowel augmentation. Five patients (3 males, 2 females), mean age years, presenting poor bladder capacity and compliance after complete exstrophy repair, underwent bladder.

To assess the feasibility of seeding adipose-derived stem cells (ADSCs) onto bladder acellular matrix grafts (BAMGs) for bladder reconstruction in a rabbit model. Autologous ADSCs were isolated, expanded and identified by flow cytometry.

In the experimental group, ADSCs were seeded onto BAMGS for reconstructing bladder defects in 12 male rabbits. Construction of the urinary bladder de novo using tissue engineering technologies is the “holy grail” of reconstructive urology.

The search for the ideal biomaterial for urinary bladder reconstruction has been ongoing for decades. One of the most promising biomaterials for this purpose seems to be bladder acellular matrix (BAM).

Further development of collagenoplasty requires addressing the problems of allergic complications, improvement of structure and maximizing therapeutic effects against pathological processes.

Keywords: Collagen, natural polymer, reconstructed collagen, decellularized collagen matrix. Roula Antoon, Herman Yeger, Yasir Loai, Syed Islam, Walid A.

Farhat, Impact of bladder‐derived acellular matrix, growth factors, and extracellular matrix constituents on the survival and multipotency of marrow‐derived mesenchymal stem cells, Journal of Biomedical Materials Research Part A, /jbm.a, A, 1, (), ().().

CK: Preparation, characterization and in vitro cytotoxicity of paclitaxel-loaded sterically stabilized solid lipid nanoparticles. Biomaterials (). Development and characterisation of a full-thickness acellular porcine bladder matrix for tissue engineering.DEVELOPMENT OF ACELLULAR MATRIX DERIVED FROM RAT’S URINARY BLADDER TO URINARY TRACT RECONSTRUCTION.

Hypothesis / aims of study This study aims to develop a decellularization protocol for urinary bladder of rat and to produce an acellular matrix that can be used as a biological scaffold.

Study design, materials and methods.

19614 views Tuesday, November 24, 2020